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Comparative Study of Two Different Centrifugal Evaporators in the Commercial Preparation of Dye Labelled Oligonucleotides.
There are many applications in modern molecular biology that require dye labelled oligonucleotides. These include “scorpion” probes1, taqman probes2 and molecular beacons3 all used in SNP genotyping. Since these are predominately used as diagnostic tools, it is of vital importance that they are of high quality. While it is easy to carry out quality control throughout the production process, once the oligonucleotide has been dried ready for shipment the only means of Q.C. is to resuspend the product. This is obviously undesirable. It is therefore important that the drying process is not detrimental to the oligonucleotide. In this study, the stability of dye labelled oligonucleotides to withstand drying in two different centrifugal evaporators was investigated.